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eukaryotic expression vector psectag2b  (Thermo Fisher)


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    Structured Review

    Thermo Fisher eukaryotic expression vector psectag2b
    Eukaryotic Expression Vector Psectag2b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psectag2b+expression+vector/pm37752138-83-4-8
    Average 86 stars, based on 1 article reviews
    eukaryotic expression vector psectag2b - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Amplification:

    Article Title: Structural basis for receptor recognition of vitamin-B(12)-intrinsic factor complexes.
    Article Snippet: Cobalamin (Cbl, vitamin B12) is a bacterial organic compound and an essential coenzyme in mammals, which take it up from the diet.. This occurs by the combined action of the gastric intrinsic factor (IF) and the ileal endocytic cubam receptor formed by the 460-kilodalton (kDa) protein cubilin and the 45-kDa transmembrane protein amnionless.. Loss of function of any of these proteins ultimately leads to Cbl deficiency in man.

    Article Title: Functional Organization of the Sortilin Vps10p Domain
    Article Snippet: .. Following amplification by PCR using appropriate oligonucleotides, constructs encoding receptors without signal and propeptide were inserted into the pSecTag2B expression vector comprising a murine Ig chain signal peptide (Invitrogen). .. The expression vector pcDNA4/Myc-HisC (Invitrogen) was used for the remaining SorCS3 constructs, and all other constructs were inserted in pcDNA3.1/Zeo( ) (Invitrogen).

    Polymerase Chain Reaction:

    Article Title: Structural basis for receptor recognition of vitamin-B(12)-intrinsic factor complexes.
    Article Snippet: Cobalamin (Cbl, vitamin B12) is a bacterial organic compound and an essential coenzyme in mammals, which take it up from the diet.. This occurs by the combined action of the gastric intrinsic factor (IF) and the ileal endocytic cubam receptor formed by the 460-kilodalton (kDa) protein cubilin and the 45-kDa transmembrane protein amnionless.. Loss of function of any of these proteins ultimately leads to Cbl deficiency in man.

    Article Title: Functional Organization of the Sortilin Vps10p Domain
    Article Snippet: .. Following amplification by PCR using appropriate oligonucleotides, constructs encoding receptors without signal and propeptide were inserted into the pSecTag2B expression vector comprising a murine Ig chain signal peptide (Invitrogen). .. The expression vector pcDNA4/Myc-HisC (Invitrogen) was used for the remaining SorCS3 constructs, and all other constructs were inserted in pcDNA3.1/Zeo( ) (Invitrogen).

    Article Title: Dimerization of the Alzheimer's disease pathogenic receptor SORLA regulates its association with retromer.
    Article Snippet: .. The PCR product was cloned into a Psectag2B expression vector (#V90020, Invitrogen) using BamHI and EcoRI restriction enzymes. ..

    Purification:

    Article Title: Structural basis for receptor recognition of vitamin-B(12)-intrinsic factor complexes.
    Article Snippet: Cobalamin (Cbl, vitamin B12) is a bacterial organic compound and an essential coenzyme in mammals, which take it up from the diet.. This occurs by the combined action of the gastric intrinsic factor (IF) and the ileal endocytic cubam receptor formed by the 460-kilodalton (kDa) protein cubilin and the 45-kDa transmembrane protein amnionless.. Loss of function of any of these proteins ultimately leads to Cbl deficiency in man.

    Gel Extraction:

    Article Title: Structural basis for receptor recognition of vitamin-B(12)-intrinsic factor complexes.
    Article Snippet: Cobalamin (Cbl, vitamin B12) is a bacterial organic compound and an essential coenzyme in mammals, which take it up from the diet.. This occurs by the combined action of the gastric intrinsic factor (IF) and the ileal endocytic cubam receptor formed by the 460-kilodalton (kDa) protein cubilin and the 45-kDa transmembrane protein amnionless.. Loss of function of any of these proteins ultimately leads to Cbl deficiency in man.

    Expressing:

    Article Title: Structural basis for receptor recognition of vitamin-B(12)-intrinsic factor complexes.
    Article Snippet: Cobalamin (Cbl, vitamin B12) is a bacterial organic compound and an essential coenzyme in mammals, which take it up from the diet.. This occurs by the combined action of the gastric intrinsic factor (IF) and the ileal endocytic cubam receptor formed by the 460-kilodalton (kDa) protein cubilin and the 45-kDa transmembrane protein amnionless.. Loss of function of any of these proteins ultimately leads to Cbl deficiency in man.

    Article Title: A Potent Germline-like Human Monoclonal Antibody Targets a pH-Sensitive Epitope on H7N9 Influenza Hemagglutinin
    Article Snippet: The baculovirus-expressed H1N1 (A/California/04/2009), H3N2 (A/Aichi/2/1968), H5N1 (A/Hongkong/213/2003), H7N7 (A/Netherlands/219/03), H7N9 (A/Shanghai/1/2013, A/Shanghai/2/2013), H10N8 (A/Jiangxi-Donghu/346/2013) and the human cell-expressed H15N8 (A/duck/AUS/341/1983) HA proteins were purchased from Sinobiological Inc. (Beijing, China). .. The HA1 gene of A/Shanghai/2/2013 was synthesized (Genscript, Nanjing, China), fused with the Fc fragment of human IgG1 and a C-terminal Avi-tag (GLNDIFEAQKIEWHE), and cloned into pSecTag2B expression vector (Thermo Fisher). .. The recombinant plasmid HA1-Fc-Avi was transfected into Expi293 cells (Thermo Fisher) for transient expression and used for biopanning.

    Article Title: Functional Organization of the Sortilin Vps10p Domain
    Article Snippet: .. Following amplification by PCR using appropriate oligonucleotides, constructs encoding receptors without signal and propeptide were inserted into the pSecTag2B expression vector comprising a murine Ig chain signal peptide (Invitrogen). .. The expression vector pcDNA4/Myc-HisC (Invitrogen) was used for the remaining SorCS3 constructs, and all other constructs were inserted in pcDNA3.1/Zeo( ) (Invitrogen).

    Article Title: Dimerization of the Alzheimer's disease pathogenic receptor SORLA regulates its association with retromer.
    Article Snippet: .. The PCR product was cloned into a Psectag2B expression vector (#V90020, Invitrogen) using BamHI and EcoRI restriction enzymes. ..

    Plasmid Preparation:

    Article Title: Structural basis for receptor recognition of vitamin-B(12)-intrinsic factor complexes.
    Article Snippet: Cobalamin (Cbl, vitamin B12) is a bacterial organic compound and an essential coenzyme in mammals, which take it up from the diet.. This occurs by the combined action of the gastric intrinsic factor (IF) and the ileal endocytic cubam receptor formed by the 460-kilodalton (kDa) protein cubilin and the 45-kDa transmembrane protein amnionless.. Loss of function of any of these proteins ultimately leads to Cbl deficiency in man.

    Article Title: A Potent Germline-like Human Monoclonal Antibody Targets a pH-Sensitive Epitope on H7N9 Influenza Hemagglutinin
    Article Snippet: The baculovirus-expressed H1N1 (A/California/04/2009), H3N2 (A/Aichi/2/1968), H5N1 (A/Hongkong/213/2003), H7N7 (A/Netherlands/219/03), H7N9 (A/Shanghai/1/2013, A/Shanghai/2/2013), H10N8 (A/Jiangxi-Donghu/346/2013) and the human cell-expressed H15N8 (A/duck/AUS/341/1983) HA proteins were purchased from Sinobiological Inc. (Beijing, China). .. The HA1 gene of A/Shanghai/2/2013 was synthesized (Genscript, Nanjing, China), fused with the Fc fragment of human IgG1 and a C-terminal Avi-tag (GLNDIFEAQKIEWHE), and cloned into pSecTag2B expression vector (Thermo Fisher). .. The recombinant plasmid HA1-Fc-Avi was transfected into Expi293 cells (Thermo Fisher) for transient expression and used for biopanning.

    Article Title: Dimerization of the Alzheimer's disease pathogenic receptor SORLA regulates its association with retromer.
    Article Snippet: .. The PCR product was cloned into a Psectag2B expression vector (#V90020, Invitrogen) using BamHI and EcoRI restriction enzymes. ..

    Synthesized:

    Article Title: A Potent Germline-like Human Monoclonal Antibody Targets a pH-Sensitive Epitope on H7N9 Influenza Hemagglutinin
    Article Snippet: The baculovirus-expressed H1N1 (A/California/04/2009), H3N2 (A/Aichi/2/1968), H5N1 (A/Hongkong/213/2003), H7N7 (A/Netherlands/219/03), H7N9 (A/Shanghai/1/2013, A/Shanghai/2/2013), H10N8 (A/Jiangxi-Donghu/346/2013) and the human cell-expressed H15N8 (A/duck/AUS/341/1983) HA proteins were purchased from Sinobiological Inc. (Beijing, China). .. The HA1 gene of A/Shanghai/2/2013 was synthesized (Genscript, Nanjing, China), fused with the Fc fragment of human IgG1 and a C-terminal Avi-tag (GLNDIFEAQKIEWHE), and cloned into pSecTag2B expression vector (Thermo Fisher). .. The recombinant plasmid HA1-Fc-Avi was transfected into Expi293 cells (Thermo Fisher) for transient expression and used for biopanning.

    Clone Assay:

    Article Title: A Potent Germline-like Human Monoclonal Antibody Targets a pH-Sensitive Epitope on H7N9 Influenza Hemagglutinin
    Article Snippet: The baculovirus-expressed H1N1 (A/California/04/2009), H3N2 (A/Aichi/2/1968), H5N1 (A/Hongkong/213/2003), H7N7 (A/Netherlands/219/03), H7N9 (A/Shanghai/1/2013, A/Shanghai/2/2013), H10N8 (A/Jiangxi-Donghu/346/2013) and the human cell-expressed H15N8 (A/duck/AUS/341/1983) HA proteins were purchased from Sinobiological Inc. (Beijing, China). .. The HA1 gene of A/Shanghai/2/2013 was synthesized (Genscript, Nanjing, China), fused with the Fc fragment of human IgG1 and a C-terminal Avi-tag (GLNDIFEAQKIEWHE), and cloned into pSecTag2B expression vector (Thermo Fisher). .. The recombinant plasmid HA1-Fc-Avi was transfected into Expi293 cells (Thermo Fisher) for transient expression and used for biopanning.

    Article Title: Dimerization of the Alzheimer's disease pathogenic receptor SORLA regulates its association with retromer.
    Article Snippet: .. The PCR product was cloned into a Psectag2B expression vector (#V90020, Invitrogen) using BamHI and EcoRI restriction enzymes. ..

    Construct:

    Article Title: Functional Organization of the Sortilin Vps10p Domain
    Article Snippet: .. Following amplification by PCR using appropriate oligonucleotides, constructs encoding receptors without signal and propeptide were inserted into the pSecTag2B expression vector comprising a murine Ig chain signal peptide (Invitrogen). .. The expression vector pcDNA4/Myc-HisC (Invitrogen) was used for the remaining SorCS3 constructs, and all other constructs were inserted in pcDNA3.1/Zeo( ) (Invitrogen).



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